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Stimulated Raman scattering microscopy by nyquist modulation of a two-branch ultrafast fiber source

A highly stable setup for stimulated Raman scattering (SRS) microscopy is presented. It is based on a two-branch femtosecond Er:fiber laser operating at a 40 MHz repetition rate. One of the outputs is directly modulated at the Nyquist frequency with an integrated electro-optic modulator (EOM). This compact source combines a jitter-free pulse synchronization with a broad tunability and allows for shot-noise limited SRS detection. The performance of the SRS microscope is illustrated with measurements on samples from material science and cell biology.

C. Riek, C. Kocher, P. Zirak, C. Kölbl, P. Fimpel, A. Leitenstorfer, A. Zumbusch, and D. Brida
Opt. Lett. 41 3731-3734 (2016)
DOI: 10.1364/OL.41.003731